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KMID : 0384119950150040594
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Korean Journal of Clinical Pathology
1995 Volume.15 No. 4 p.594 ~ p.603
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Comparison of Cultrue, Acid-Fast Stain and Polymerase Chain Reaction Assay for Detection of Mycobacterium Tuberculosis
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염양숙
정옥연/장숙진/문대수/박영진
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Abstract
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Background :
@EN Broad apphcation of rapid and sensitive methods for the detection and identification of Mycobacterium tuberculosis(M. tuberculosis) is essential to control the spread of mycobacterial infection. This report compared the results of acid-fast
stains
by traditional concentration and cytocentrifugation method, culture and polymerase chain reaction (PCR) assay for the direct detection of M. tuberculosis.
@ES Methods :
@EN Acid-fast stains by traditional concentration and cytocentrifugation method, culture and PCR assay from 61 sputum specimens were processed according to the standard laboratory procedure. The specimens were liquefied and decontaminated by
N-acetyl-L
cysteine-NaOH(NAIC-NaOH) method. The specimens for the Acid-fast stains were sterilized by 5% sodium hypochlorite. In the PCR assay, a 123-bp segment within the M. tuberculosis specific insertion sequence IS6110 was amplified.
@ES Results :
@EN Comprehensive evaluation of all records of patients such as all laboratory datas, chest x-ray finding and clinical features revealed that 24(39.3%) of 61 sputum specimens were obtained from confirmed tuberculosis patients. On the basis of
this
conclusion, following statistical parameters were evaluated ; overall sensitivity, specificity, positive predictive value, negative predictive value and efficiency of the tests, and which were 91.7, 100, 100, 94.9 and 96.7% respectively for the
culture
method ; 54.2, 100, 100, 77.1 and 82.0% for Acid fast stain by the traditional concentration method ; 79.1, 97.3, 95.0, 87.8 and 90.2% for Acid-fast stain by the cytocentrufugation ; 91.7, 89.2, 84.6, 94.3 and 90.2% for the PCR assay ;
@ES Conclusion :
@EN The PCR assay is fast, efficient and sensitive method which can detect M. tuberculosis in sputum directly. The Acid-fast stain by cytocentrifugation is simple, fast, sensitive and efficient method to detect M. tuberculosis in sputum(Korean J
Clin
Pathol 1995 ; 15(4) : 594~603).
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